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IHC

What is Immunohistochemistry?

About this technology

What is Immunohistochemistry?

is a well-established highly specific and sensitive method that allows detection and localization of specific antigens with sub-cellular level resolution. It also offers detection of molecular and morphological changes in formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections or any other cytological preparations. The technique has proved invaluable in research and diagnostic areas for researchers and pathologists, respectively, where it can assist with accurate tumor classification and diagnosis. BioGenex offers IHC ancillaries to improve sensitivity, enhance staining quality, and minimize background noise.

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IHC Detection Kits

Detection Systems are specially designed for the chromogenic detection of antigen-antibody binding reactions with primary antibodies of various sources (mouse or rabbit, IgG or IgM). BioGenex IHC Detection Kits are developed for high sensitivity and robust staining with low background and are compatible with both automated platforms and manual staining procedures.


BioGenex is pleased to introduce Rapid One Step Poly-HRP Rabbit detection kit with unmatched sensitivity and clean intense stain with the shortest assay protocol. This system use a non-streptavidin-biotin proprietary micro polymer-complex technology to minimize background staining wherein the Polymer-HRP reagent is bound to the primary antibody and visualized by diaminobenzidine (DAB).

Features and Benefits:

  • Proprietary micro polymer-complex technology
  • High sensitivity for less abundant antigen detection
  • Specific and separate systems for anti-mouse and anti-rabbit antibodies
  • Highly specific for nuclear, cytoplasmic, and membrane antigen staining
  • Clean, crisp, and reproducible results
  • Short protocol and fast turnaround time

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BioGenex Super Sensitive™ Polymer-HRP Detection System uses a non-streptavidin-biotin proprietary micro polymer-complex technology to minimize background staining wherein an antibody enhancer/amplifier and a polymer-HRP reagent are bound to the primary antibody and visualized by diaminobenzidine (DAB).

Features and Benefits:

  • Proprietary micro polymer-complex technology
  • High signal-to-noise ratio without endogenous biotin background
  • Excellent sensitivity for low abundant proteins
  • Universal system for rabbit and mouse antibodies
  • Excellent penetration ability for intense nuclear, cytoplasmic, and membrane antigen staining
  • Enabling higher dilution of antibodies for reduced cost

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BioGenex New & Improved Super Sensitive™ 1-Step Polymer-HRP Detection System uses a non-streptavidin-biotin based proprietary micro polymer-complex technology to minimize background staining. The Polymer-HRP conjugated secondary antibody binds to the primary antibody and is visualized by diaminobenzidine (DAB).

Features and Benefits:

  • Proprietary micro polymer-complex technology
  • High sensitivity for low abundant proteins
  • Universal system for rabbit and mouse antibodies
  • Robust and specific staining for nuclear, cytoplasmic, and membrane antigens
  • Clean, crisp, and reproducible results
  • Short protocol and fast turnaround time

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BioGenex offers the New & Improved Poly-HRP Super Sensitive™ detection system, which uses a non-streptavidin-biotin based proprietary micro polymer-complex technology to minimize the background staining. This kit enables the chromogenic detection of antigen-antibody binding reactions with unmatched sensitivity and clean and intense staining with the shortest assay protocol.

Features and Benefits:

  • Fast 40 min turn-around time
  • Universal system for rabbit and mouse antibodies
  • Unmatched sensitivity allows for higher antibody dilution and cost reduction
  • Robust and specific staining for low abundant nuclear, cytoplasmic, and membrane antigens

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Super Sensitive™ (SS) Link-Label IHC Detection System is a classic system based on specific and sensitive streptavidin-biotin interaction to detect a bound primary antibody. These kits include multi-Link – a mix of anti-mouse and anti-rabbit IgGs conjugated to multiple biotin molecules, and a Label – Streptavidin conjugated with an enzyme Alkaline Phosphatase (AP).

Features and Benefits:

  • Improved staining of weak antigens
  • User-friendly and extensive choice of kit formats designed for use with human, animal, or rodent tissues
  • A wide choice of chromogens offered
  • Intense staining and reproducible results

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BioGenex Super Sensitive™ (SS) Link-Label IHC Detection System is a classic system based on highly specific and sensitive streptavidin-biotin interaction to detect a bound primary antibody. These kits include multi-Link – a mix of anti-mouse and anti-rabbit IgGs conjugated to multiple biotin molecules, and a Label – Streptavidin conjugated with an enzyme Horseradish peroxidase (HRP).

Features and Benefits:

  • Improved staining against weak antigenicity
  • User-friendly and extensive choice of kit formats designed for use with human, animal, or rodent tissues
  • Intense staining and reproducible results

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The BioGenex Super Sensitive™ Mouse on Mouse 1-step HRP IHC Detection System represents state of the art technology in the detection of antigen-antibody binding reactions on mouse tissue. This kit is specifically designed to localize mouse primary antibodies on mouse tissues. The Mouse on Mouse kit can be used with normal and genetically engineered mouse models, including xenograft, transgenic, knock out and other mutant strains.

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Primary Antibodies

BioGenex offers more than 600 primary antibodies with a specific emphasis on cancer and infectious diseases. All monoclonal and polyclonal antibodies are quality controlled and highly optimized to provide a maximum signal with the minimum background when used for immunohistochemical staining on formalin-fixed, paraffin-embedded (FFPE) tissue.

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IHC Panel Markers

BioGenex offers a wide range of IHC antibodies for tumor diagnosis and categorization (initial differentiation, tumor origin, and prognosis). All BioGenex antibodies are validated on human tissues to ensure sensitivity and specificity. Comprehensive IHC Panels include a range of monoclonal and polyclonal antibodies of mouse and rabbit origin.

IHC Ancillaries

BioGenex is a one-stop solution for molecular pathology reagents and automated workstations. We offer high-quality products to help our customers generate reproducible and reliable tissue stainings. Our comprehensive suite of ancillary supplies and consumables include Antigen Retrieval solutions, Enzymes for Tissue Digestion, Dewaxing, Blocking reagents, Buffers and Diluents, Counter Stains and Mounting media, Vials, Pipette tips, Slides, Coverslips, and Pap pens.


BioGenex has developed solutions for antigen retrieval, decal retrieval in decalcified tissues, and nucleic acid retrieval for in situ hybridization (ISH) applications. BioGenex’s new EZ-AR™ Elegance retrieval solutions eliminate common issues such as improper heat distribution, buffer evaporation, tissue drying, and boil-overs during antigen retrieval. Using EZ-AR™ Elegance solutions with the EZ-Retriever® Microwave System streamline the retrieval processes, including deparaffinization, rehydration, and antigen retrieval.

Antigen retrieval procedure

The quality of the staining results depends on adherence to the antigen retrieval protocol. Briefly, the reagent containers are firstly filled with antigen retrieval solution, followed by placing the rack of slides into the reagent containers and then placing them inside the EZ-Retriever® Microwave System for treatment.

Enzymes for Tissue Processing

Enzymes– Immunoreactivity of an epitope can be recovered using by proteolytic-induced epitope retrieval (PIER). PIER is an enzymatic method of antigen retrieval which relies on enzymes such as proteinase K, trypsin, or pepsin to unmask the antigenic epitopes by cleaving peptides.

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Buffers and diluents are available for immunohistochemistry, in situ hybridization and most other applications.

  • General buffers, such as PBS (pH 7.6) and TBS (pH 7.6, 0.1M) can be used for washing/rinsing of slides.
  • Super Sensitive™ Wash Buffer is a phosphate buffered saline (pH 7.4) with surfactant and is used to ensure optimal staining with even spreading of antibodies and other reagents to avoid inconsistent results.

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BioGenex provides several substrate pack options both for manual and automated systems for in situ detection using alkaline phospahatase and peroxidase enzymes. The kits are designed to reduce substrate incubation times and minimize exposure to chemical hazards. These substrate packs allow microscopic visualization of different cellular components depending on the marker used either by in situ hybridization (ISH) or immunohistochemistry (IHC).

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BioGenex offers the following counterstains for use in Immunohistochemistry, in situ Hybridization and other applications with either manual or automated staining systems.

Counterstain

Mayer’s hematoxylin is a blue stain that does not contain alcohol and therefore is compatible with both alcohol soluble non-permanent chromogens (AEC, Fast Red and New Fuchsin) and alcohol-insoluble chromogens (DAB and Elegance Red). It is alcohol and xylene insoluble and therefore compatible with most clearing agents and mounting media.

Mounting

Mounting of all stained biological specimens is an essential step before their microscopic evaluation. Mounting also enables the slides to be archived for long periods of time. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term or short-term preservation is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

  • SuperMount® Permanent Mounting Medium is a polymer based aqueous mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex’s U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears.
  • Aqueous Mounting Medium is glycerol-based mounting medium that require the use of a coverslip. It is intended for short-term specimen storage and is compatible with most chromogens and counterstains.
  • XMount™ Mounting Medium is a permanent mounting medium that has been optimized for use with BioGenex Xmatrx® instrument for all BioGenex detection systems for immunohistochemistry (IHC), In Situ Hybridization (ISH) and special stains. XMount™ is intended for use with alcohol and xylene insoluble chromogens, such as DAB (for peroxidase systems) and Elegance Red (for alkaline phosphatase systems). XMount dries clear with an ideal refractive index similar to high quality glass and tissue elements. Mounted slides can be viewed with high magnification oil immersion lenses. Also, when mounting preparations stained with the BCIP/NBT substrate, crystal formation that may occur when using other media is minimized.

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  • Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.
  • Power Block™: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power Block reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium.
  • Protein Block: This can be used to reduce background staining due to non-specific binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody.

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BioGenex Provides concentrated and RTU Link-Label IHC Detection System based on the highly specific and sensitive streptavidin-biotin interaction to detect a bound primary antibody. These kits include multi-Link – a mix of anti-mouse and anti-rabbit IgGs conjugated to multiple biotin molecules and a Label – Streptavidin conjugated with an enzyme (Horseradish peroxidase (HRP) or Alkaline Phosphatase (AP).

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BioGenex provides high-quality products which are aimed at accelerating the generation of reproducible and reliable tissue staining results. The consumables, we offer include Vials, Pipette tips, Slides, Coverslips, Pap pen.

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