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FISH

What is Fluorescent in situ hybridization?

About this technology

What is Fluorescent in situ hybridization?

Fluorescent in situ hybridization (FISH) is an important method for genetic mapping and gene expression profiling. This technique exploits the use of fluorescent dye-labeled probes against a nucleotide sequence of DNA that is mapped or localized to a chromosome or intra-nuclear DNA to generate a fluorescence signal which in turn is visualized in situ by microscopy. The hybridization of the probe to the corresponding nucleotide sequence enables detection of the presence or absence of specific sequences, mapping of translocation break points, and determination of gene copy numbers. Automated FISH diagnostic systems have been approved by the US FDA under the medical device stream, a mark of the maturity and reliability of the technique.

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Multiplex Detection Kits

eFISH Visualization kit is intended for use in a fluorescence in situ hybridization (FISH) procedure and is optimized to detect target nucleic acid sequences. It is designed to detect fluorescent-labeled nucleic acid probes following their hybridization to the target DNA or RNA sequences.
eFISH Visualization kits are available for formalin-fixed paraffin-embedded (FFPE) tissue sections and cell preparations. BioGenex visualization kits provide unsurpassed performance.

Features and Benefits

  • Complete solution for eFISH assays on tissue and cell
  • Universal system for all fluorescent labeled eFISH probes
  • Available for manual and automated applications
  • High signal to noise ratio for intense fluorescent signals
  • Clean, crisp and reproducible results
  • Can be used with third party eFISH probes

The BioGenex eFISH cytology kit is a novel system for processing of eFISH assay of cytological/hematological smearsorcells. This kit contains reagents for pretreatment and post-hybridization stringency washes of the tissue. This kit is to be used for BioGenex eFISH processing platforms Xmatrx® Infinity/Elite, Xmatrx® NANO, NANO VIP and Xmatrx® MINI along with BioGenex eFISH probes.

Features and Benefits:

  • Proprietary micro polymer-complex technology
  • High sensitivity for less abundant antigen detection
  • Specific and separate systems for anti-mouse and anti-rabbit antibodies
  • Highly specific for nuclear, cytoplasmic, and membrane antigen staining
  • Clean, crisp, and reproducible results
  • Short protocol and fast turnaround time

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The BioGenex eFISH Histo kit is a novel system for processing eFISH assay on formalin-fixed, paraffin-embedded (FFPE) tissue. This kit contains reagents for pretreatment and post-hybridization stringency washes. These kits are designed for use on BioGenex eFISH platforms Xmatrx® Infinity/ELITE, Xmatrx® NANO, NANO VIP and Xmatrx® MINI along with BioGenex eFISH probes.

Features and Benefits:

  • Proprietary micro polymer-complex technology
  • High sensitivity for less abundant antigen detection
  • Specific and separate systems for anti-mouse and anti-rabbit antibodies
  • Highly specific for nuclear, cytoplasmic, and membrane antigen staining
  • Clean, crisp, and reproducible results
  • Short protocol and fast turnaround time

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eFISH Probes

In the eFISH procedure, fixed tissue sections are pretreated to expose target DNA or RNA sequences. Appropriately labeled probes hybridize to the exposed target DNA or RNA sequences in cells, and the following stringent washing steps remove non-specific binding. Subsequently, slides are mounted using DAPI/antifade and can be visualized under a fluorescence microscope using the appropriate filter set. BioGenex provides a whole catalog of eFISH probes as well as automated systems

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eFISH Ancillaries

BioGenex is a one-stop solution for molecular pathology reagents and automated workstations. We offer high-quality products to help our customers generate reproducible and reliable tissue stainings. Our comprehensive suite of ancillary supplies and consumables include Antigen Retrieval solutions, Enzymes for Tissue Digestion, Dewaxing reagents, Mounting media, Vials, Pipette tips, Slides, Coverslips, and Pap pens


BioGenex has developed solutions for antigen retrieval, decal retrieval in decalcified tissues, and nucleic acid retrieval for in situ hybridization (ISH) applications. BioGenex’s new EZ-AR™ Elegance retrieval solutions eliminate common issues such as improper heat distribution, buffer evaporation, tissue drying, and boil-overs during antigen retrieval. Using EZ-AR™ Elegance solutions with the EZ-Retriever® Microwave System streamline the retrieval processes, including deparaffinization, rehydration, and antigen retrieval.

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BioGenex offers the following counterstains for use in Immunohistochemistry, in situ Hybridization and other applications with either manual or automated staining systems.

Counterstain

Mayer’s hematoxylin is a blue stain that does not contain alcohol and therefore is compatible with both alcohol soluble non-permanent chromogens (AEC, Fast Red and New Fuchsin) and alcohol-insoluble chromogens (DAB and Elegance Red). It is alcohol and xylene insoluble and therefore compatible with most clearing agents and mounting media.

Mounting

Mounting of all stained biological specimens is an essential step before their microscopic evaluation. Mounting also enables the slides to be archived for long periods of time. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term or short-term preservation is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

  • SuperMount® Permanent Mounting Medium is a polymer based aqueous mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex’s U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears.
  • Aqueous Mounting Medium is glycerol-based mounting medium that require the use of a coverslip. It is intended for short-term specimen storage and is compatible with most chromogens and counterstains.
  • XMount™ Mounting Medium is a permanent mounting medium that has been optimized for use with BioGenex Xmatrx® instrument for all BioGenex detection systems for immunohistochemistry (IHC), In Situ Hybridization (ISH) and special stains. XMount™ is intended for use with alcohol and xylene insoluble chromogens, such as DAB (for peroxidase systems) and Elegance Red (for alkaline phosphatase systems). XMount dries clear with an ideal refractive index similar to high quality glass and tissue elements. Mounted slides can be viewed with high magnification oil immersion lenses. Also, when mounting preparations stained with the BCIP/NBT substrate, crystal formation that may occur when using other media is minimized.

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BioGenex provides high-quality products which are aimed at accelerating the generation of reproducible and reliable tissue staining results. The consumables, we offer include Vials, Pipette tips, Slides, Coverslips, Pap pen.

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