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Double Staining

What is Double Staining?

About this technology

What is Double Staining?

BioGenex Double Staining Super Sensitive™ Polymer-HRP detection kits are designed using novel polymer-HRP technology to simultaneously detect multiple antigens on a single slide with fast and easy IHC protocols. This double staining system is optimized for human tissues with superior sensitivity and specificity to produce precise and reliable results assisting in rapid and accurate diagnosis

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Double Staining Detection Kits

BioGenex Double Staining Super Sensitive™ Polymer-HRP detection kits are designed using novel polymer-HRP technology to simultaneously detect multiple antigens on a single slide with fast and easy IHC protocols. This double staining system is optimized for human tissues with superior sensitivity and specificity to produce precise and reliable results assisting in rapid and accurate diagnosis

Features and Benefits

  • Visualize Multiple IHC markers simultaneously
  • Enhance productivity, minimize reagent & labor cost
  • Reduce turnaround-time vis-à-vis traditional sequential staining
  • Clean, Intense and reproducible staining

Primary Antibodies

BioGenex offers more than 600 primary antibodies with a specific emphasis on cancer and infectious diseases. All monoclonal and polyclonal antibodies are quality controlled and highly optimized to provide a maximum signal with the minimum background when used for immunohistochemical staining on formalin-fixed, paraffin-embedded (FFPE) tissue.

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Double Staining Ancillaries

Ancillaries as a one-stop-shop provider of molecular pathology workstations and reagents, we also offer a comprehensive suite of ancillary products to support our customers. These high-quality products are aimed at accelerating the generation of reproducible and reliable tissue staining results. The ancillaries and consumables, we offer include Antigen Retrievals, Enzymes for Tissue Digestion, Dewaxing and Blocking reagents, Buffers and Diluents, Counter Stains and Mounting media, Vials, Pipette tips, Slides, Coverslips, Pap pen.


BioGenex has developed solutions for antigen retrieval, decal retrieval in decalcified tissues, and nucleic acid retrieval for in situ hybridization (ISH) applications. BioGenex’s new EZ-AR™ Elegance retrieval solutions eliminate common issues such as improper heat distribution, buffer evaporation, tissue drying, and boil-overs during antigen retrieval. Using EZ-AR™ Elegance solutions with the EZ-Retriever® Microwave System streamline the retrieval processes, including deparaffinization, rehydration, and antigen retrieval.

Antigen retrieval procedure

The quality of the staining results depends on adherence to the antigen retrieval protocol. Briefly, the reagent containers are firstly filled with antigen retrieval solution, followed by placing the rack of slides into the reagent containers and then placing them inside the EZ-Retriever® Microwave System for treatment.

Enzymes for Tissue Processing

Enzymes– Immunoreactivity of an epitope can be recovered using by proteolytic-induced epitope retrieval (PIER). PIER is an enzymatic method of antigen retrieval which relies on enzymes such as proteinase K, trypsin, or pepsin to unmask the antigenic epitopes by cleaving peptides.

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Buffers and diluents are available for immunohistochemistry, in situ hybridization and most other applications.

  • General buffers, such as PBS (pH 7.6) and TBS (pH 7.6, 0.1M) can be used for washing/rinsing of slides.
  • Super Sensitive™ Wash Buffer is a phosphate buffered saline (pH 7.4) with surfactant and is used to ensure optimal staining with even spreading of antibodies and other reagents to avoid inconsistent results.

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BioGenex provides several substrate pack options both for manual and automated systems for in situ detection using alkaline phospahatase and peroxidase enzymes. The kits are designed to reduce substrate incubation times and minimize exposure to chemical hazards. These substrate packs allow microscopic visualization of different cellular components depending on the marker used either by in situ hybridization (ISH) or immunohistochemistry (IHC).

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BioGenex offers the following counterstains for use in Immunohistochemistry, in situ Hybridization and other applications with either manual or automated staining systems.

Counterstain

Mayer’s hematoxylin is a blue stain that does not contain alcohol and therefore is compatible with both alcohol soluble non-permanent chromogens (AEC, Fast Red and New Fuchsin) and alcohol-insoluble chromogens (DAB and Elegance Red). It is alcohol and xylene insoluble and therefore compatible with most clearing agents and mounting media.

Mounting

Mounting of all stained biological specimens is an essential step before their microscopic evaluation. Mounting also enables the slides to be archived for long periods of time. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term or short-term preservation is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

  • SuperMount® Permanent Mounting Medium is a polymer based aqueous mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex’s U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears.
  • Aqueous Mounting Medium is glycerol-based mounting medium that require the use of a coverslip. It is intended for short-term specimen storage and is compatible with most chromogens and counterstains.
  • XMount™ Mounting Medium is a permanent mounting medium that has been optimized for use with BioGenex Xmatrx® instrument for all BioGenex detection systems for immunohistochemistry (IHC), In Situ Hybridization (ISH) and special stains. XMount™ is intended for use with alcohol and xylene insoluble chromogens, such as DAB (for peroxidase systems) and Elegance Red (for alkaline phosphatase systems). XMount dries clear with an ideal refractive index similar to high quality glass and tissue elements. Mounted slides can be viewed with high magnification oil immersion lenses. Also, when mounting preparations stained with the BCIP/NBT substrate, crystal formation that may occur when using other media is minimized.

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  • Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.
  • Power Block™: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power Block reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium.
  • Protein Block: This can be used to reduce background staining due to non-specific binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody.

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BioGenex provides high-quality products which are aimed at accelerating the generation of reproducible and reliable tissue staining results. The consumables, we offer include Vials, Pipette tips, Slides, Coverslips, Pap pen.

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