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Instrumentation

What is continuous access?

Most autostainers on the market do not allow for the addition of new samples for staining when a run is in progress. Xmatrx Diagnostics breaks this limitation by enabling continuous operation of the system. The user can add new slides in the middle of a run if empty slide positions are available. The slides that are loaded in continuous mode will be processed along with the current slides without affecting the current run. Continuous Access enables the user to remove completed slides and load new ones without the need to wait for run completion. In addition, reagents that are no longer required can be removed and new ones can be added.

What is STAT access? How is it different from continuous access?

STAT (Latin: statum, meaning: to rush, urgent) Access allows the addition of emergency specimen slides for staining during an ongoing run. STAT slides will be processed and completed at the highest priority. During the processing of STAT slides, all other slides being processed shall remain on hold and are buffered regularly to keep the tissue hydrated. After completion of the STAT slides, all pending slides that are on hold will be processed according to their protocol.

What is the microwave pressure cooker protocol and what are its advantages?

The microwave pressure cooker method is a procedure that utilizes a microwave with a pressure cooker for antigen retrieval and staining. The pressure cooker sold by BioGenex is especially designed for use in a microwave oven. Its advantages include the speed of treatment, reproducibility of results with large batches of slides, ability to use metal slide racks, and economy of equipment costs.

How do I determine which antigen retrieval solution to use with my antibody?

BioGenex provides information on the specific antigen retrieval solution recommended for use with its primary antibodies. We offer Retrievit Sampler Kit for use with primary antibodies from other manufacturers. This kit provides the customer with five different antigen retrieval solutions so the user can determine which one is the best for that particular antibody.

Antibodies and Detection Kits

Can all the antibodies be used on tissues of different species?

BioGenex antibodies are validated and intended for use on human FFPE and frozen tissues. If used on tissues from other species, the protocol must be validated by the end user.

What kind of detection kits can be used for most antibodies?

All of our antibodies can be used with the following detection kits provided by BioGenex:

Super Sensitive Polymer-IHC Detection System
Super Sensitive One-Step Polymer-HRP Detection System
Super Sensitive Link-Label IHC Detection System

Can BioGenex antibodies be used for automated and manual staining?

Yes, most of our antibodies come in manual and automation packs and can be used for both purposes.

Can BioGenex Detection kits be used with other vendor’s antibodies?

Yes, BioGenex detection kits are universal detection systems for mouse and rabbit antibodies.

Can BioGenex antibodies be used for other applications such as ELISA and Southern Blotting?

BioGenex antibodies are tested and validated for IHC. For any procedure or technique apart from IHC, the protocol must be validated by the user.

Can the detection kits be used on tissues from other species?

The detection kits are designed for use in human tissue. However, the Super SensitiveTM Link-Label IHC detection system comes in a choice of kit formats designed for use with human, animal or rodent tissue.

What is the shelf life of your antibodies?

Two years from date of manufacture when stored according to the product information page.

What is the difference between one-step and two step-detection kit?

The one-step detection kit is designed with proprietary technology which eliminates the use of Super Enhancer required by the two-step detection kit. Therefore, the one-step kit provides superior sensitivity and specificity with the advantage of a short protocol.

How would I choose the correct secondary antibodies?

Most primary antibodies raised in mouse (IgG +IgM) and rabbit (IgG) work with our Poly-HRP Secondary antibody. In case of primary antibodies raised in other species, we also provide species-specific secondary antibodies for use with our Link-Label System.

Why is an antibody discontinued? Do you have any replacement for the same?

There are various reasons as to why we discontinue certain antibodies ranging from raw material issues to insufficient sales. The replacement products, if available, can be provided on request.

Where can I find the protein concentration of BioGenex antibody?

For concentrated antibodies, the protein concentration is available on the data sheet for that specific antibody. Ready-to-Use (RTU) antibodies are pre-diluted for optimal staining results. We can provide the concentration of RTU antibodies upon request.

Ancillary Reagents

What is the difference between the normal antigen retrieval and EZ Elegance antigen retrieval solutions?

The proprietary technology used in the EZ-AR Elegance solutions provides stronger intensity and reproducible results. These solutions are designed for used with automated platforms such as Xmatrx systems and the EZ Retriever. The normal antigen solutions are intended for use with manual protocols.

Is antigen retrieval required for frozen and de-calcified tissues?

The requirement of antigen retrieval depends on the antibody used and its staining protocol. For frozen and de-calcified tissues, heat-based antigen retrieval is NOT recommended. We provide alternate solutions for these kinds of tissues such as the Decal Retrieval Solution for acid-decalcified bone marrow tissue and enzymes for tissue digestion.

Which chromogen can be used with which antibody?

Most antibodies can be used either with DAB or AEC chromogen depending on the preference for staining under a light microscope.

Would the chromogen be based on mounting technique?

The choice of mounting medium following immunochemical staining is largely dictated by the chromogen label used to visualize the antigen. Compatible Mounting Media for Chromogens and Counterstains

CounterstainsAECBCIP/NBTDABElegance RedFast RedNew Fuchsin
HematoxylinAqueous/ SuperMountAqueous /Not recommendedAqueous/ Permanent/ SuperMountAqueous/ Permanent/ SuperMountSuperMount / AqueousSuperMount
Methyl GreenNot recommendedPermanentPermanentPermanentPermanentPermanent
Nuclear Fast RedNot recommendedAqueous/ Permanent/ SuperMountAqueous/ Permanent/ SuperMountNot recommendedNot recommendedNot recommended
Ancillary Reagents

What is the difference between the normal antigen retrieval and EZ Elegance antigen retrieval solutions?

The proprietary technology used in the EZ-AR Elegance solutions provides stronger intensity and reproducible results. These solutions are designed for used with automated platforms such as Xmatrx systems and the EZ Retriever. The normal antigen solutions are intended for use with manual protocols.

Is antigen retrieval required for frozen and de-calcified tissues?

The requirement of antigen retrieval depends on the antibody used and its staining protocol. For frozen and de-calcified tissues, heat-based antigen retrieval is NOT recommended. We provide alternate solutions for these kinds of tissues such as the Decal Retrieval Solution for acid-decalcified bone marrow tissue and enzymes for tissue digestion.

Which chromogen can be used with which antibody?

Most antibodies can be used either with DAB or AEC chromogen depending on the preference for staining under a light microscope.

Would the chromogen be based on mounting technique?

The choice of mounting medium following immunochemical staining is largely dictated by the chromogen label used to visualize the antigen. Compatible Mounting Media for Chromogens and Counterstains

CounterstainsAECBCIP/NBTDABElegance RedFast RedNew Fuchsin
HematoxylinAqueous/ SuperMountAqueous /Not recommendedAqueous/ Permanent/ SuperMountAqueous/ Permanent/ SuperMountSuperMount / AqueousSuperMount
Methyl GreenNot recommendedPermanentPermanentPermanentPermanentPermanent
Nuclear Fast RedNot recommendedAqueous/ Permanent/ SuperMountAqueous/ Permanent/ SuperMountNot recommendedNot recommendedNot recommended
ISH Probes and Detection Kits

Can the probes from BioGenex be used for fluorescent staining?

BioGenex probes are intended to use with Super SensitiveTM One Step ISH detection kit and optimized for chromogenic visualization under bright field microscopy. However, if the end-user wants to use any other kit and visualization technique, they need to validate their own protocol.

Which detection kit can be used with these probes?

We provide a universal detection kit for all probes (Super SensitiveTM One-Step ISH detection kit).

What kind of tissue specimens can be used with these probes?

FFPE tissues, pap smears and cell smudges can be used with these probes.

Xmatrx Elite and Infinity

Who will install the instrument?

BioGenex field service engineer will install the instrument. Your local sales repsentative and application scientist will be involved in training and optimizing your protocols on the instrument.

Do I need to calibrate my instrument every time I use it?

No you do not need to calibrate the instrument every time you use it. The instrument will be calibrated when it is installed. Re-calibration will be required if the instrument is moved to a different location.

Does the instrument need to be under a hood?

No the instrument does not need to be under a hood.

Do I need some special location to place my instrument (Lab Bench)?

Xmatrx Elite / Infinity is provided with a standalone systems. So the user will not require any extra bench space. The unit will be received as a cabinet with all the bulk reagents placed within it.

How do I clean my instrument?

This includes just a basic cleaning of the instrument to prevent any contamination and to ensure proper functionality of the instrument. We recommend basic cleaning done by the user as per the Maintenance Chart.

How many times in a year will a PM to be done on my instrument?

BioGenex field Service engineer / trained distributor will perform Two PM’s per year

Whom do I need to call if I have questions on my XmatrxSystem?

BioGenex Technical Support @ 1 510 824 1400 should be able to answer all your technical questions or customerservice@biogenex.com; or indiacs@biogenex.com or internationalcs@biogenex.com

You may also contact your local Sales Representative OR/and Application Specialist

What is the allowed temperature range for the instrument?

The best allowed temperature range for the instrument is 15°C to 30°C.

How do I load my slides on the Xmatrx system?

BioGenex has designed the Slide racks for the Xmatrx System. The slides just need to be place into the Slide holder slot and then close the clip.

Are the slide racks easy to load and unload?

Yes they just need to be placed in the slots on the base of the instrument.

How do I buy the BioGenex Consumables to be run on the Xmatrx?

You can order them through Customer Service +1 510 824 1400or email to customerservice@biogenex.com; or indiacs@biogenex.com or internationalcs@biogenex.com

Can I bake my slides on the instrument?

Yes, the slides can be baked on the Xmatrx System @ 60°C for 1 hour and also you have the flexibility to adjust the Temperature and Time. Apart from this the protocol can be used for baking along with instrument starts and finishing the run.

Can I use Xylene on the instrument?

No.  Xylene is not recommended, better to use BioGenex De-waxing solution.

Can I do Antigen Retrieval on the system?

Yes you can do antigen retrieval on the system.

Can I have different temperatures for each of my protocols?

Yes. The slide heaters are individually temperature controlled.

How long does the unit take to heat up to 95°C and cool down to Room Temperature?

< 2 Minutes

Can I use my own Antibodies?

BioGenex sells most of the antibody panels that are required in a clinical lab. In case, if any antibody is not available fromBioGenex, the User will have an option to buy an empty Barcoded vial to run their own Antibody for Elite. In Case of Infinity instrument 3rd Party Antibody can be used without the Barcoded vial.

What are Barcoded reagents?

This allows the system to track reagent usage, lot numbers and expiry to ensure that the correct reagent is being used for Xmatrx Elite only.

Do I need to always use BioGenex reagents?

BioGenexsells Xviz detection system, which is be used to run all antibodies. The Xmatrx Elite is a closed system, which ensures that the user will have the best and optimal results using BioGenex detection systems. Xmatrx Infinity is an Open System, all the Detection kit and Antibodies can be used as per customer choice.

Is it possible to buy more reagent racks?

Yes. Please contact BioGenex’s, Customer Service @ +1 510 824 1400 

What happens when the system does not read my slide barcode label?

When the system does not read the slide barcode label, the user may manually select the protocol from the internal database of BioGenex protocols.

What happens when the system does not read Barcode Label on my reagent vial?

When the system does not read the Reagent Vial, user may have the provision to add manually (enter the serial number) by selecting the reagent from the list. A reagent vial is entered into the list either or when the system reads the Barcoded vial for the first time. If the reagent does not exist in the database, the vial will have to be returned back to BioGenex, to be replaced by a new vial. Please contact BioGenex Technical Support @ 1 800 421 4149 for more information.

What is the dead volume of reagent vials?

Different vial types have different dead volumes:

  • 17 ml vials have a dead volume of 400ul
  • 2 ml vials  for ISH have a dead volume of 50ul 

What is the minimum reagent volume that can be used on the Xmatrx?

Xmatrx system can be programmed to use as little as 10ul. However, different applications & operations may have different minimums. Please refer to the protocol datasheet for the volume information on a specific protocol.

What is the maximum reagent volume that can be used on the Xmatrx?

Xmatrx system can be programmed to use as much as 850ul. However, different applications & operations may have different maximums. Please refer to the protocol datasheet for volume information on a specific protocol.

Can I create a new reagent?

Elite: No. The Xmatrx Elite is a closed system, which ensures that the user will have the best and optimal results using BioGenex detection systems.

Infinity: Yes, it’s open System.Can create a New Reagent, Antibody or increase the number of steps in the protocol or can create new Protocols also.

Do I need to order pipette tips from BioGenex?

Yes, for Xmatrx we have Consumable kit which has Barrier Slides, Small and Large Pipette tips and coverslips.

Please contact BioGenex, Customer Service @ 1 800 421 4149. 

When will the small pipette tips be used?

While processing expensive reagents like AR Solution, Antibody/Probe, Xmatrx system uses small tips. Typically, volume range for this step is 10-100ul.

When will the large pipette tips be used?

While applying large volumes (more than 100ul), Xmatrx system uses large tips. Typically, volume range for this step is 100-850ul. 

Can I reuse the pipette tips and Coverslips?

No. System discards the tips and Coverslips after use. Reuse of these discarded tips and Coverslips is not recommended, as it will cause cross contamination and also damages the instrument functionality.

What is the size of Coverslips

Xmatrx system is designed to work with 3 different sizes of Coverslips:

  • 18 x 18 mm
  • 25 x 25 mm
  • 25 x 40 mm

 

Where do I order the reagents for my bulk bottles?

Yes,pleasecontact BioGenex, Customer Service @ 1 800 421 4149.

What is the dead volume of the bulk bottles?

Approximately 100 ml

Do I need to fill all the bulk bottles, even if my application does not need all buffers?

No. Xmatrx system displays the required buffers in the pre-run prime window. Disconnect the unused buffer lines from the system before starting a run.

When do I need to prime the bulk reagents?

It is recommended that you prime the required solutions before every run for consistent staining quality. If it is not possible, you may want to prime the solutions whenever you make up new reagents or operate the system after a long period of inactivity (e.g., 2-3 days). This eliminates the air bubbles in the buffer lines and ensures continuous flow of the buffer solutions.

Does Xmatrxä mount my slides?

Yes the Xmatrxä System will mount the slides using permanent mounting media. (Xmountä) Once the mounting media is applied it will also add the Coverslip

How should the waste be disposed?

There are Two8 liter waste containers attached to the Xmatrx Instrument and for disposing the Waste need to follow the “Waste Management Process” specified as per the LAB standards or follow  any regular biohazard waste Procedure

Can I use any standard slide or do I have to purchase BGX slide only?

Xmatrx System requires a Special Micro Chamber Slides with hydrophobic barrier, after oil sealing it canwith-stand longer incubation time and very minimal reagent usage. These slides are in 3 different Patterns (18 x 18mm, 25 x 25mm and 25 x 40mm). These slides need to be purchased from BioGenex only.

Can I use consumables (Coverslips, Pipette tip & Slides) from different vendor?

No. All consumables must be purchased from BioGenex only

How flexible is the XMatrx software?

Infinity: Xmatrx allows you to create your own protocols in addition to BioGenex supplied ones.

Elite: Being a closed system, Xmatrx Elite requires you to run all the protocols (BioGenex and User created) in a closed format. Once a protocol is scanned into a slide position, you can edit the allowed fields, if needed. Allowed fields are: Temperature, Incubation, Buffer Selection, number of Count &Micro Chamber sealing.

Can we do remote diagnostics with XMatrx?

Each PC that ships with XMatrx will be configured for remote access capability. If a PC is connected to Internet, with required access rights, remote diagnostic can be performed. 

How often do I need to calibrate the instrument?

Instrument is calibrated before shipping out. While setting up the system, calibration must be checked. Once setup, re-calibration is not required for day-to-day operation. PM will address the calibration issues, if any.

How frequently new software versions will be released & how will they affect the previous releases?

Need for updates/bug fixes, if any, determines the frequency of new software releases.  There could be 1-2 releases every 10 – 12 months.

How do you explain the user friendliness of the software?

Graphical User Interface for Xmatrx is based on our popular i6000 interface. Xmatrx GUI expands the i6000 interface to enrich your programming experience. Main interface window displays the slide map and control panel. Slide map offers an intuitive and convenient way of selecting & editing slide protocols and to query the progress of a slide during the run.

Various operations are color-coded. Slide objects on the main window are displayed in appropriate colors based on their current operation making it convenient to get a snapshot of the run status from a distance. In the event of an error condition, system plays out an appropriate audio message and also visual message on the control panel.

Which Operating systems are supported for Xmatrx?

XMatrx is developed and tested on Windows XP / Win 7 and Win 10. Currently providing with Win 10 Operating System

Does instrument requires the Backup UPS?

Yes, either needs to connect to the Centralized UPS or the Separate UPS with 1500Watts. If the Power Failure rate is more then, it’s better to connect to longer UPS Back up.

Whether UPS will be shipped along with the Instrument?

No, it’s always better to arrange from the User End.

Is Instrument can be connected to 110 V / 230V?

Yes, without any change user can plug the instrument power to 110V or 230V power Supply from UPS.

Is Earth Voltage important for the instrument?

Yes, the Neutral to Earth Voltage should be always less than 2 Volts (AC) for the Medical instruments.

What are the instrument dimensions in D X W X H inches?

Dimensions are 29 x 66 x 59 Inches

What is the Instrument total weight?

400 lb /182 Kg

Does the barcode for the Detection kit can be printed?

Elite: No, only the Slides labels can be printed with i500 printer, whereas the Detection kit needs to be purchased from BioGenex only.

Xmatrx Infinity has Barcode to read slides and Reagents vials?

No, Infinity will proceed without the barcodes slides and reagents scanning

Xmatrx Elite can Perform FISH Application?

Yes, it will be added based on Special request from customers-end and you need to contact BioGenex, Customer Service @ 1 800 421 4149, if not then FISH cannot be performed on Elite System.

Reagents
What is Immunohistochemistry?

Immunohistochemistry is a well-established, highly specific and sensitive method that allows the localization of an antigen within a cell or any other biological tissue samples with high resolution. The bound antigen-antibody complexes are then visualized using chemically linked enzymes, dyes, or fluorescent labels.

What is the purpose of Immunohistochemistry?

Immunohistochemistry is used to help diagnose diseases, such as cancer. It may also be used to tell the difference between different types of cancer.

What are the common steps of Immunohistochemistry?

  1. Baking
  2. Deparaffinization and rehydration
  3. Antigen Retrieval
  4. Peroxide Blocking/Endogenous peroxidaseblocking
  5. Power Blocking/Protein Blocking
  6. Primary Antibody
  7. Super Enhancer/Linker
  8. Poly-HRP/ Secondary Antibody/ Label
  9. DAB
  10. Counter staining
  11. Dehydration, Clearing and mounting

Note– If one is using one step poly HRP then “vii” point is not applicable.

What is used to preserve tissues for IHC staining?

10% Neutral Buffered Formalin

What is the tissue thickness generally required for IHC?

3-5µ

What is commonly used to remove paraffin from IHC slides?

Biogenex provides Dewax solution for deparaffinization. It is a non-toxic solution. Commonly Xylene is used but it is a toxic solution.

What is the purpose of antigen retrieval?

The process of sample fixation can lead to protein cross-linking, which masks antigens and can restrict antigen-antibody binding. Antigen retrieval enables an antibody to access the target protein within the tissue.

What are the methods of antigen retrieval?

  1. Enzymatic/proteolytic antigen retrieval
  2. Heat-induced antigen retrieval method

What is Polyclonal antibody?

These are antibodies against many epitopes of one antigen

What is Monoclonal Antibody?

These are antibodies against single epitope of single antigen

What is antigen?

These are foreign molecules to which the body may be exposed and respond with a reaction specifically and bind to the antigen. These are typically proteins or polysaccharides within or on the surface of cell.

What are some of the most popular ways of visualizing antibody-antigen interaction?

Antibody is conjugated to an enzyme, such as peroxidase, that can catalyze a color-producing reaction. Antibody can be tagged to a fluorophore, such as fluorescein (immunofluorescence)

What are the common enzymes used in immunohistochemistry?

  • Horseradish peroxidase (HRP)
  • Alkaline phosphatase

What is the purpose of using ISH/In Situ Hybridization?

In Situ Hybridization (ISH) is a powerful technique for precise detection and localization of a specific nucleic acid sequence within a histologic section.

What is the underlying principle behind ISH?

The nucleic acids within a histologic specimen can be detected by the hybridization of a complementary nucleic acid probe in which a reporter molecule is attached. Common non-radioactive labels for probe are fluorescein and digoxigenin. Subsequent visualization of the reporter molecule localizes DNA or RNA sequences in tissue or cells samples.

What are the different approaches of ISH?

There are two basic approaches to visualize the DNA or RNA target sequences in situ: chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). While these approaches involve the same underlying principle, the instrumentation required to visualize the specimen is different.

Which instruments are used for ISH visualization?

  • Chromogenic ISH: Bright field microscope
  • FISH: Fluorescence microscope

What are the uses of ISH & FISH?

ISH- ISH has broad applications such as physical chromosome mapping, karyotyping, and pathogen profiling. Riboprobe also enables the localization and analysis of gene expression profile.

FISH- As a diagnostic tool, FISH is widely used in clinical genetics, neuroscience, reproductive medicine, toxicology, microbial ecology, evolutionary biology, comparative genomics, cellular genomics and chromosome biology to check for genetic abnormalities associated with prenatal diagnosis, cancer, birth defects and mental retardation. It is possible to differentiate benign and malignant tumors in the same tissue using FISH.

What are the product lines of BioGenex?

  1. Dewaxing/Deparaffinization solution
  2. Buffer and enzymes for antigen retrieval
  3. Buffers and consumables like tips, coated slides, vials
  4. Antibodies
  5. IHC Detection kits
  6. Mounting media
  7. ISH probes, miRNA probes
  8. ISH detection kits
  9. FISH probes
  10. FISH detection kits
  11. Blocking Reagents
  12. Automated instrument for IHC, ISH, FISH
  13. Covid-19 RT-PCR detection kit
  14. VTM

What are the type of antibodies does BioGenex provide?

  • AC (Antibody Cocktails)
  • AM (Mouse Monoclonal Antibodies)
  • AN (Rabbit Monoclonal Antibodies)
  • AX, AY (Monoclonal Antibodies for Xmatrx®)
  • AW (Polyclonal Antibodies for Xmatrx®)
  • AR (Polyclonal Antibodies)
  • MU (Mouse Monoclonal Antibodies)
  • NU (Rabbit Monoclonal Antibodies)
  • PU (Polyclonal Antibodies)

What are the pack sizes of BioGenex manual detection kits for IHC?

  • 60tests
  • 500tests
  • 1000test

 
What are the research instruments does BioGenex offer?

  1. Xmatrx Infinity
  2. i6000 Infinity
  3. NANO VIP
  4. Xmatrx NANO
  5. Xmatrx MINI

What are the diagnostics instruments does BioGenex offer?

  1. Xmatrx Elite
  2. i6000 Diagnostics

What is the use of i500 plus?

It is used for Barcodedslide label printing

What is the slide capacity of NANO VIP?

10

What is the difference between NANO VIP and Xmatrx NANO?

NANO VIP is a complete automatic instrument but Xmatrx NANO is a semi-automatic one where the reagent dispensing is manual. Rest of the functions is similar

What are the kits available for FISH at BioGenex?

  1. eFISH Histo (DF500-20X)
  2. eFISH Cyto (DF510-20X)

What are the FISH probe pack sizes does BioGenex offer?

10 tests, 20 tests

What is the pack size of FISH kits?

20 tests

What are the BioGenex instruments that can perform FISH?

  1. Xmatrx MINI
  2. Xmatrx NANO
  3. NANO VIP
  4. Xmatrx Elite
  5. Xmatrx Infinity

What is the workstation instrument does BioGenex provide?

Xmatrx MINI

How many primary antibodies does Biogenex provide?

550+

What is the mechanism of EZ-Retriever?

Microwave

What is the runtime for EZ-Retriever?

30min for 96 slides

What is the operational temperature and humidity of i6000?

  • Temperature: 15-30oC
  • Humidity: 15-55% RH

Are we able to perform cell preparations, smears and fine needle aspirates (FNAs) on i6000?

Yes

Does i6000 have liquid level sensors?

Yes

Does i6000 diagnostic allow using 3rd party antibodies?

Yes

How many slides can we perform on Xmatrx Elite in a day?

100 slides per day (60 slides in eight-hour shift and 40 slides in delayed overnight run)

How one can use 3rd party antibody on Xmatrx Elite?

We are providing User Defined Barcoded Empty vials. The customer has to buy those to run 3rd party antibodies.

Do we need to set any protocol for IHC on Xmatrx Elite?

No, it is not required. The system has already 400+ preloaded protocols and protocols for 3rd party antibodies. One can only change/edit incubation time and wash number and times.

Can we retrieve experimental data from Xmatrx?

Yes

What is the mechanism used on Xmatrx during reagent incubation?

Microchamber

Can we perform FISH and ISH on i6000?

No

Is there any provision to use insert vials in i6000?

Yes

Can we perform ICC (Immunocytochemistry) on i6000?

Yes

Is there any provision to use insert vials in Xmatrx?

Yes

Can anyone use delayed run option on Xmatrx, i6000 or NANO VIP?

Yes

Can we use frozen tissue on Xmatrx or NANO VIP?

Yes, we can however some steps need to be modified by the customer.

Do I need to cut my sections on special slides to run on the Xmatrx system?

Yes, you need to take barrier slides from BioGenex specially design for the Xmatrx to create microchamber.

Do the tissues need to be embedded in special paraffin to be used on the Xmatrx?

You can use any of the standard paraffin wax.

Do I have to do On-line Dewaxing?

You can use dewaxing online/offline. It’s up to your convenience.

Can I use Xylene and alcohol on the instrument?

You can use alcohol on the instrument. We do not recommend use of Xylene on automation.

Can I do Antigen Retrieval on the system?

Yes you can do antigen retrieval on system (Xmatrx, Xmatrx MINI, NANO, and NANO VIP). On i6000 you cannot do antigen retrieval on instrument. For i6000, you will need BioGenex EZ-RT system.

What solution do I need to use for Antigen Retrieval?

EZ-AR1/AR2 Elegance on automation and EZ-RT system and EZ-AR 1/AR 2 for manual and EZ-RT system.

Can I use third party Antibodies?

Yes, in open system you can use third party/your antibodies. In closed systems like Xmatrx Elite and i6000 Diagnostics, there is a provision to use third party/own antibodies on BioGenex BARCODED USER DEFINED vials.

What are Barcoded reagents?

Barcoded reagents are for use with close system. After reading the barcode, instrument will starts the run. It helps in removing the human errors. Accurate & precise real-time reagent tracking on Xmatrx and i6000 is done using barcode.

Do I need to always use BioGenex’s reagents?

For close system you need to use only Biogenex products because we have a no. of standardized protocol for IHC, ISH and FISH in close system.

What is the dead volume of reagent vials?

For Xmatrx vial it is 400ul, for i6000 vial it is 500ul, for NanoVIP vial it is 400ul.

What is the minimum reagent volume in the vials that can be used on the Xmatrx?

1ml

What is the maximum reagent volume in the vials that can be used on the Xmatrx?

16ml

Can I reuse the pipette tips?

No

Can I reuse the cover-slips?

No

Do I need to fill all buffer carboys, even if my application does not need all buffers?

No, you can fill only the required buffer carboys.

When do I need to prime the bulk reagents?.

Just before starting the run.

Ques What are the types of detection systems I need to use on Xmatrx?.

QD550-YCDE, QD550-YCXE, QD610-YADEN

How do I store the mounting media?

At 4°C

Will the mounting media not solidify?

At 4°C it will not solidify.

Does Xmatrx produce any Toxic waste?

Yes, it produces DAB waste, and it is carcinogenic.

How should the waste be disposed?

Do as recommended by chemical waste disposal authority.

What are the different applications that can be run on the Xmatrx?

IHC, ISH, FISH, IF, ICC

Performance related issues like No/low staining?

Check the antibody dilution, incubation time, other reagents and protocol for the particular antibody.

Protein and IgG concentration?

For RTU 1-3 µg/ul

How to perform double staining with our antibodies?

You can use BioGenex double staining kit or you can use one poly HRP and second Poly AP antibody for mouse and rabbit antibody respectively.

Difference between polymer and link label?

Polymer HRP is streptavidin and biotin free and link –label contains streptavidin and biotin.

What is clear mount and X-mount?

Clear mount is Aquous and X-mount is non-Aquous.

Does Biogenex antibodies functional on Western Blot?

Yes, antibodies may work for western blot.

Can we use BioGenex detection system and antibodies on frozen sections?

Yes

What is the  pH of different AR-solutions?

Citric acid based AR solution pH is around 6.0-6.2 and Tris EDTA based AR solution pH is around 9.0.

BioGenex antibodies work on Cell smears or not and if yes what is the protocol?

Antibodies works well on cell smears but one has to standardize the protocol for the same.

Do we need to wash with DI or buffer after DAB step?

Wash with DI water

What is the difference between power and protein block?

Power block is based on BSA and protein block is based on Horse serum.

Is Sodium azide present in antibody?

Yes

Does BioGenex antibodies works on Ventana detection system or not?

A few of the antibodies work on Ventana.

What is avidin/Biotin Block?

Avidin/Biotin Block blocks all endogenous biotin, biotin receptors, and avidin binding sites present in tissues.

Can we use BioGenex antibodies in indirect immunofluorescence?

Yes, customer will have to standardize the protocol for the same.

What is the name of rinse buffer used after dewax solution?

It is a super sensitive wash buffer.

BioGenex antibodies suspend in which liquid. Do they contain latex in it?

BioGenex antibodies are suspended in antibody diluent contains BSA. It does not contain latex.

What is the recommended volume range for Antibody that is used in IHC on Xmatrx  Elite?

100-120µL

Which nomenclature is used to denote the Ready to Use 6 ml Antibody for the manual use?

AM/AN/AR-XXX-5M

What are the recommended barrier slides for performing IHC protocols on Xmatrx Elite?

25X40 Barrier slides

How many slides can be performed in a single run on EZ-Retriever for Antigen Retrieval ?

96 Slides

The temperature ranges on EZ-retriever system

Ambient to 107oC

How many Reagent vials can be loaded in the Reagent rack of Xmatrx Elite for performing IHC?

49 Reagents

The binding site of Antibody is known as

Epitope

Which operations during IHC cannot be performed on i6000?

Baking, deparaffinization, Antigen retrieval and final coverslipping.

  • EZ-retriever can perform
  • De-Waxing, Rehydration, Antigen retrieval

The reagents dispense volume ranges on i6000

100ul -1000ul

The time required attaining the ambient to 95oC temperature on Xmatrx during the heating of slide

<1min

Reagent Rack & vial capacity on i6000 Diagnostics & i6000 infinity is common. Pls specify the combination

2 rack X30 vials

The total slide capacity on Xmatrx Elite/Infinity is

40 Slides

What are the slide patterns available on i6000 for performing the real runs?

1/3rd test area, 2/3rd test area, Full test area

Accurate & precise real-time reagent tracking on Xmatrx and i6000 Diagnosticsis done using

Barcode

What is the total slide capacity on the i6000 instrument?

60 Slides at a time

How many Reagent positions are available on the i6000 instrument?

60 reagents

What type of kit can be used on i6000 Diagnostics?

QD410-YCDE

What is the minimum and maximum temperature range on Xmatrx?

Ambient – 105⁰C

How many continuous slides can be incorporated on i6000 Diagnostics?

20 Slides

How many STAT samples can be performed in a single run on i6000 Diagnostics?

6 Slides

What is the function of EZ-Dewax?

Deparaffinization, Rehydration

How many times we can use continuous feature on i6000 Diagnostics?

2 Attempts

What is the total capacity of the waste carboy in use on Xmatrx?

8 Liters

What is the total capacity of the buffer carboys on Xmatrx?

4 Liters

What is the ideal pressure for operation of Xmatrx?

5±0.5Pa

How is the reagent vial detected on i6000 Diagnostics?

Barcode

What is the fill volume of i6000 OptiMiser Reagent vials?

20mL

What are the formats of BioGenex antibodies for sale?

AXXXX-5ME-6ml, AXXXX-10ME-10ml, AXXXX-YCDE-16ml, XU328-UCE-1 ml, XU328-5UCE-0.5ml

What is the charge onslides used in IHC procedure?

Positive

What is used as counterstain in IHC procedure?

Hematoxylin

What is the standard temp for baking of tissue slides of IHC?

60oC for 1 hr

What is the difference between one-step and two-step kits?

Both super enhancer and Poly-HRP is used in two step kit and One step kit do not have super enhancer.

How many individual protocols can be performed in one single run on Xmatrx?

40 Individual protocols can be performed in one single run on Xmatrx