Detection Systems – ISH

The in situ Hybridization (ISH) is a powerful technique for detecting and localizing specific nucleic acid sequences within cells or tissues. This is achieved by the hybridization of a labeled probe to the specific RNA/DNA sequence within the cell and subsequent detection of the bound probe. ISH technique enables the semi-quantification of mRNA expression and helps determine the temporal and spatial patterns of gene expression in cells, tissue and whole animals. ISH technique can also be used for detection of intracellular pathogens with a very high degree of sensitivity.

Super Sensitive™ One-Step Polymer-HRP Detection System

BioGenex Super Sensitive™ One-Step Polymer-HRP ISH Detection Kit is suited specifically for chromogenic ISH (CISH) assays. It uses a non-streptavidin-biotin proprietary micropolymer-complex technology to minimize background staining while providing superb probe detection.The in situ Hybridization (ISH) is a powerful technique for detecting and localizing specific nucleic acid sequenceswithin cells or tissues. This is achieved by the hybridization of a labeled probe to the specific RNA/DNA sequence within the cell and subsequent detection of the bound probeThere are two basic approaches to visualize the DNA or RNA target sequences in situ: chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). BioGenex Super Sensitive™ One-Step Polymer-HRP ISH Detection is specifically designed for CISH.

Features and Benefits

• Highly Sensitive and specific for chromogenic RNA/DNA detecion
• Visualize single copy gene
• Can be used with third party probes
• Clean, Intense and reproducible staining.