Ki-67 is expressed during all phases of the cell cycle except Go. Expression of Ki-67 antigen closely parallels [3 H]-thymidine incorporation, a standard method of measuring cell proliferation rate. Ki67 antigen is a potent tool for evaluating the growth fraction of any given human cell subset, especially malignant tumors and other pathogenic states, as a measure of the proportion of proliferating cells. Immunostaining of Ki67 antigen in normal tissue shows nuclear reactivity in cells of germinal centers of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, and undifferentiated spermatogonia. Resting cells such as lymphocytes, monocytes, parietal cells and Paneth’s cells of gastrointestinal mucosa, hepatocytes, renal cells, and mature sperm cells did not stain.
|Tissue Type/Cancer Type|
0.5 mL – Manual – Concentrate, 1 mL – Manual – Concentrate, 6 mL – Manual – RTU, 50 Tests – Automation – Xmatrx, 100 Tests – Automation – i6000, 200 Tests – Automation – Xmatrx, 5 slides – Xmatrx, 5 slides – Manual