Clone 100 reacts with bcl-2 alpha oncoprotein as assessed by reaction with a 26-kD band in immunoblotting. This antibody stains the 380 cell line which carries a 14;18 chromosomal translocation. bcl-2 oncogene codes for two closely similar proteins, bcl-2 alpha and bcl-2 beta. These two proteins differ only in their carboxyl terminal sequences. bcl-2 is an integral inner mitochondrial membrane protein and is frequently overexpressed in many lymphoid malignancies. This protein also interferes with programmed cell death independent of promoting cell division. Immunohistologic studies have demonstrated that staining for bcl-2 protein can be used to distinguish neoplastic germinal centers from reactive ones.
5 slides – Manual, 5 slides – Xmatrx