ISH Enzymes
- Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.
- Power Block™: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power Block reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium.
- Enzymes– Immunoreactivity of an epitope can be recovered using by proteolytic-induced epitope retrieval (PIER). PIER is an enzymatic method of antigen retrieval which relies on enzymes such as proteinase K, and pepsin to unmask the antigenic epitopes by cleaving peptides.
