Ancillaries & Consumables

Ancillaries as a one-stop-shop provider of molecular pathology workstations and reagents, we also offer a comprehensive suite of ancillary products to support our customers. These high-quality products are aimed at accelerating the generation of reproducible and reliable tissue staining results. The ancillaries and consumables, we offer include Antigen Retrievals, Enzymes for Tissue Digestion, Dewaxing and Blocking reagents, Buffers and Diluents, Counter Stains and Mounting media, Vials, Pipette tips, Slides, Coverslips, Pap pen.

Antigen Retrieval & Dewax Products
Enzymes for Tissue Processing
Dewaxing & Rehydration Reagents
Blocking Reagents

Blocking Reagents

  • Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.
  • Power Block™: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power Block reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium.
  • Protein Block: This can be used to reduce background staining due to non-specific binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody.

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Buffers and Diluents

Buffers and Diluents

Buffers and diluents are available for immunohistochemistry, in situ hybridization and most other applications.

  • General buffers, such as PBS (pH 7.6) and TBS (pH 7.6, 0.1M) can be used for washing/rinsing of slides.
  • Super Sensitive™ Wash Buffer is a phosphate buffered saline (pH 7.4) with surfactant and is used to ensure optimal staining with even spreading of antibodies and other reagents to avoid inconsistent results.

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Links / Labels - Manual

BioGenex offers the following counterstains for use in Immunohistochemistry, in situ Hybridization and other applications with either manual or automated staining systems.

  • Mayer’s hematoxylin is a blue stain that does not contain alcohol and therefore is compatible with both alcohol soluble non-permanent chromogens (AEC, Fast Red and New Fuchsin) and alcohol-insoluble chromogens (DAB and Elegance Red). It is alcohol and xylene insoluble and therefore compatible with most clearing agents and mounting media.

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Substrates and Chromogens
Mounting Media
Mounting of all stained biological specimens is an essential step before their microscopic evaluation. Mounting also enables the slides to be archived for long periods of time. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term or short-term preservation is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

  • SuperMount® Permanent Mounting Medium is a polymer based aqueous mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex’s U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears.
  • Aqueous Mounting Medium is glycerol-based mounting medium that require the use of a coverslip. It is intended for short-term specimen storage and is compatible with most chromogens and counterstains.
  • XMount™ Mounting Medium is a permanent mounting medium that has been optimized for use with BioGenex Xmatrx® instrument for all BioGenex detection systems for immunohistochemistry (IHC), In Situ Hybridization (ISH) and special stains. XMount™ is intended for use with alcohol and xylene insoluble chromogens, such as DAB (for peroxidase systems) and Elegance Red (for alkaline phosphatase systems). XMount dries clear with an ideal refractive index similar to high quality glass and tissue elements. Mounted slides can be viewed with high magnification oil immersion lenses. Also, when mounting preparations stained with the BCIP/NBT substrate, crystal formation that may occur when using other media is minimized.

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Microscope slides and coverslips

OptiPlus™ Positive-Charged Microscope Slides provide a strong adhesive surface for tissues and cells to prevent tissue displacement during harsh pre-treatments such as enzymatic digestion and the microwave Antigen Retrieval method. These slides are ideal for automated systems. Additionally, each slide has a frosted end for easy labeling. The OptiPlus™ Positive-Charged Barrier Slides have all the advantages of our regular OptiPlus™ slides, but also contain hydrophobic barriers that allow the quantity of reagents per slide to be tailored to the size of the specimen. These slides eliminate reagent waste without the need to use a PAP pen, thereby reducing set-up time in manual assays as well as in automated systems. The permanent hydrophobic barriers are compatible with dewaxing solutions and other reagents. The slides are suitable for use with frozen tissue sections, formalin-fixed paraffin sections, and cytology preparations.

Microscope Barrier Slides & Coverslips for Xmatrx

OptiPlus™ Barrier Slides for Xmatrx® (U.S. & Foreign Equivalent Patents Pending) contain a double hydrophobic barrier that allows formation of an oil seal to prevent evaporation of micro-reagents during high temperature steps and prolonged incubations. Four different configurations are available:

  • A single test area of 25 x 40 mm (>80 µL of reagent recommended)
  • A single test area of 25 x 25 mm (>40 µL of reagent recommended)
  • A single test area of 18 x 18 mm (>10 µL of reagent recommended)
  • Two test area per slide, each measuring 18 x 18 mm

Coverslips are optimized for use on Xmatrx® staining systems and come is three configurations to accommodate the different barrier slides.

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Microscope Slides &  accesories for i6000™ and Manual

OptiPlus™ Barrier slides for i6000™ come in three different configurations to accommodate different tissue sizes or multiple tissues per slide:

  • A single, full-size test area of 25 x 40 mm
  • A single 2/3-size test area of 25 x 30 mm
  • Three 1/3-size test areas per slide, each measuring 25 x 15 mm

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Reagent vials and accessories
i6000™ Diagnostics & Xmatrx® Infinity:
The OptiMiser™ reagent vials (U.S. & Foreign Equivalent Patents Pending) are available as a 20 mL disposable pack for use on the i6000™ or Xmatrx® Infinity staining systems. Vials are designed to minimize dead volume:

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Xmatrx® Elite: Reagents vials for Xmatrx® Elite Automated Staining Systems are barcoded 17 ml vials especially designed to ensure  accurate  identification, proper reagent inventory management and staining of up to 200 slides. These vials dead volume is minimized to <0.5 ml. Barcode vials for use with antibodies from any supplier (user defined) are also available (XT077-AX601 to XT077-AX0999).

ISH probes are supplied in 2 ml vials, inserted in barcode vial holders thus minimizing dead volume to <0.05 ml. Barcode vials for use with ISH probes from any supplier (user defined) are also available (XT079-PR0050 to XT079-PR0099).

Reagent Vials & Accessories for Open system Automation

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Reagent Vials & Accessories for i6000™ Diagnostics

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Reagent Vials & Accessories for Xmatrx® Elite

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Consumables kit
Pipette tips

BioGenex pipette tips are made of high-quality polypropylene and are RNase and heavy metal-free when untampered. Inner surface is extremely smooth and requires minimum wetting. 1 mL pipette tips are optimized for use on BioGenex Xmatrx® and i6000™ Staining Systems, while 200 µL tips are optimized for Xmatrx® staining systems.

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The PAP Pen is a useful pen-like tool for immunohistochemical staining methods. It is designed to prevent the waste of valuable reagents by forming a water-repellent barrier around the specimen. This barrier creates the proper surface tension to hold an antibody solution or detection reagents within the target area on the slide. The surface tension provided by the PAP Pen circle ensures that only the amount of antibody solution needed for sufficient reaction will be applied. Since over-flooding of the slide is eliminated, wiping of excess fluid around the specimen can be avoided. The PAP Pen can be used for immunostaining of paraffin sections, frozen sections, and for fluorescent antibody methods. The PAP Pen contains a special formulation, which is water repellent. It can be removed, if desired, with xylene or xylene substitutes after the staining procedure is completed.

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