Instrumentation

  • What is RFID technology?

    Radio Frequency Identification (RFID) is a term used to define technologies that use radio waves for identification of objects. RFID system consists of a tag and a reader with antenna. Each BioGenex reagent vial is tagged with a unique radio collar that ensures the system to track reagent usage, lot numbers and safeguards against incorrect usage. This tool allows easy placement of the vials anywhere on the reagent rack since the information is stored on the RFID chip placed on each reagent vial.

  • What is the difference between RFID and barcode technology?

    RFID and barcode technology differ in line of sight technology. For the scanner to read the barcode, the code has to be aligned towards the scanner. In contrast, RFID tags can be read as long as they are within the range of the reader. BioGenex uses RFID technology on all reagent vials to ensure real-time tracking of reagent usage. Barcode technology used on other systems only provides passive information and cannot register features such as reagent usage, remainder and tracking.

  • What is continuous access?

    Most autostainers on the market do not allow for the addition of new samples for staining when a run is in progress. Xmatrx Diagnostics breaks this limitation by enabling continuous operation of the system. The user can add new slides in the middle of a run if empty slide positions are available. The slides that are loaded in continuous mode will be processed along with the current slides without affecting the current run. Continuous Access enables the user to remove completed slides and load new ones without the need to wait for run completion. In addition, reagents that are no longer required can be removed and new ones can be added.

  • What is STAT access? How is it different from continuous access?

    STAT Access allows the addition of emergency specimen slides for staining during an ongoing run. STAT slides will be processed and completed at the highest priority. During the processing of STAT slides, all other slides being processed shall remain on hold and are buffered regularly to keep the tissue hydrated. After completion of the STAT slides, all pending slides that are on hold will be processed according to their protocol.

  • What is the microwave pressure cooker protocol and what are its advantages?

    The microwave pressure cooker method is a procedure that utilizes a microwave with a pressure cooker for antigen retrieval and staining. The pressure cooker sold by BioGenex is especially designed for use in a microwave oven. Its advantages include the speed of treatment, reproducibility of results with large batches of slides, ability to use metal slide racks, and economy of equipment costs.

  • How do I determine which antigen retrieval solution to use with my antibody?

    BioGenex provides information on the specific antigen retrieval solution recommended for use with its primary antibodies. We offer Retrievit Sampler Kit (Cat. # BK-1000-25) for use with primary antibodies from other manufacturers. This kit provides the customer with five different antigen retrieval solutions so the user can determine which one is the best for that particular antibody.

Antibodies and Detection Kits

  • Can all the antibodies be used on tissues of different species?

    BioGenex antibodies are validated and intended for use on human FFPE and frozen tissues. If used on tissues from other species, the protocol must be validated by the end user.

  • What kind of detection kits can be used for most antibodies?

    All of our antibodies can be used with the following detection kits provided by BioGenex:

    • Super Sensitive Polymer-IHC Detection System
    • Super Sensitive One-Step Polymer-HRP Detection System
    • Super Sensitive Link-Label IHC Detection System
  • Can BioGenex antibodies be used for automated and manual staining?

    Yes, most of our antibodies come in manual and automation packs and can be used for both purposes.

  • Can BioGenex Detection kits be used with other vendor’s antibodies?

    Yes, BioGenex detection kits are universal detection systems for mouse and rabbit antibodies.

  • Can BioGenex antibodies be used for other applications such as ELISA and Southern Blotting?

    BioGenex antibodies are tested and validated for IHC. For any procedure or technique apart from IHC, the protocol must be validated by the user.

  • Can the detection kits be used on tissues from other species?

    The detection kits are designed for use in human tissue. However, the Super SensitiveTM Link-Label IHC detection system comes in a choice of kit formats designed for use with human, animal or rodent tissue.

  • What is the shelf life of your antibodies?

    Two years from date of manufacture when stored according to the product information page.

  • What is the difference between one-step and two step-detection kit?

    The one-step detection kit is designed with proprietary technology which eliminates the use of Super Enhancer required by the two-step detection kit. Therefore, the one-step kit provides superior sensitivity and specificity with the advantage of a short protocol.

  • How would I choose the correct secondary antibodies?

    Most primary antibodies raised in mouse (IgG +IgM) and rabbit (IgG) work with our Poly-HRP Secondary antibody. In case of primary antibodies raised in other species, we also provide species-specific secondary antibodies for use with our Link-Label System.

  • Why is an antibody discontinued? Do you have any replacement for the same?

    There are various reasons as to why we discontinue certain antibodies ranging from raw material issues to insufficient sales. The replacement products, if available, can be provided on request.

  • Where can I find the protein concentration of BioGenex antibody?

    For concentrated antibodies, the protein concentration is available on the data sheet for that specific antibody. Ready-to-Use (RTU) antibodies are pre-diluted for optimal staining results. We can provide the concentration of RTU antibodies upon request.

Ancillary Reagents

  • What is the difference between the normal antigen retrieval and EZ elegance antigen retrieval solutions?

    The proprietary technology used in the EZ-AR Elegance solutions provides stronger intensity and reproducible results. These solutions are designed for used with automated platforms such as Xmatrx systems and the EZ Retriever. The normal antigen solutions are intended for use with manual protocols.

  • Is agricultural retrieval required for frozen and de-calcified tissues?

    The requirement of antigen retrieval depends on the antibody used and its staining protocol. For frozen and de-calcified tissues, heat-based antigen retrieval is NOT recommended. We provide alternate solutions for these kinds of tissues such as the Decal Retrieval Solution for acid-decalcified bone marrow tissue and enzymes for tissue digestion.

  • Which Chromogen can be used with which antibody?

    Most antibodies can be used either with DAB or AEC chromogen depending on the preference for staining under a light microscope.

  • Would the chromogen be based on mounting technique?

    The choice of mounting medium following immunochemical staining is largely dictated by the chromogen label used to visualize the antigen.

    Compatible Mounting Media for Chromogens and Counterstains

    Counterstains AEC BCIP/NBT DAB Elegance Red Fast Red New Fuchsin
    Hematoxylin Aqueous/ SuperMount Aqueous /Not recommended Aqueous/ Permanent/ SuperMount Aqueous/ Permanent/ SuperMount SuperMount / Aqueous SuperMount
    Methyl Green Not recommended Permanent Permanent Permanent Permanent Permanent
    Nuclear Fast Red Not recommended Aqueous/ Permanent/ SuperMount Aqueous/ Permanent/ SuperMount Not recommended Not recommended Not recommended

    a May have poor color based contrast

ISH Probes and Detection Kits

  • Can the probes from BioGenex be used for fluorescent staining?

    BioGenex probes are intended to use with Super SensitiveTM One Step ISH detection Kit and optimized for chromogenic visualization under bright field microscopy. However, if the end-user wants to use any other kit and visualization technique, they need to validate their own protocol.

  • Which detection kit can be used with these probes?

    We provide a universal detection kit for all probes (Super SensitiveTM One-Step ISH detection Kit).

  • What kind of tissue specimens can be used with these probes?

    FFPE tissues, pap smears and cell smudges can be used with these probes.