In Situ Hybridization (ISH) is a powerful technique for precise detection and localization of a specific nucleic acid sequence within a histologic section. The underlying principle of ISH is that the nucleic acids within a histologic specimen can be detected by the hybridization of a complementary nucleic acid probe in which a reporter molecule is attached. Subsequent visualization of the reporter molecule localizes DNA or RNA sequences in tissue or cells samples.
BioGenex ISH detection systems enable the semi-quantification of mRNA expression and help determine the temporal and spatial patterns of gene expression in cells and tissues. They are also used for detecting intracellular pathogens with a very high degree of sensitivity.