CISH

CISH

Chromogenic in situ hybridization (CISH) is a process in which a labeled complementary DNA or RNA strand is used to detect and localize a specific nucleic acid sequence within a histologic section (e.g. formalin-fixed, paraffin-embedded (FFPE) tissue sections, cytology specimens). Following the conventional alkaline phosphatase or peroxidase reactions, the histologic section is visualized under a standard bright-field microscope. CISH has broad applications such as mapping of translocation break points and determination of gene copy numbers. Riboprobes also enables the localization and analysis of gene expression profile

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